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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene continues to be noticed in lots of tumors, which leads to chromosome instability, and thus may well lead to tumorigenesis. This protein was found to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a important part in promoting cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are described. Two more splice variants have been claimed but thorough nucleotide sequence information will not be yet offered.

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Blocking peptides are peptides that bind especially to your goal antibody and block antibody binding. These peptide typically is made up of the epitope identified from the antibody.

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This guidebook outlines further doable optimization of cellular and immunoassay parameters to be sure the very best benefits are...

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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to guarantee the very best outcomes are...

Antibodies certain to the blocking peptide no longer bind towards the epitope around the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, one can see which staining is certain.

Target Information Cyclin click here E belongs to the really conserved cyclin spouse and children, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E sorts a fancy with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates in the G1-S stage boundary and is also degraded as cells progress by way of S period.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...

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